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KMID : 0900920020260040369
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Korean journal of Animal Reproduction
2002 Volume.26 No. 4 p.369 ~ p.375
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A Sperm Factor Inducing Second Polar Body Formation in Mouse Secondayy Oocyte
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Park Y.-S.
Min S.-H.
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Abstract
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A sperm factor(s) for oocyte activation during fertilization has not been clearly identified. In this study to elucidate an oocyte activation factor(s), mouse sperm were sonicated and ultra-filtered with a 30 kilo-daltons (KD) cutoff membrane and the ultra-filtrate was then sequentially fractionated over Suporose 12 column and Superdex column, The recovered fractions were micro-injected into Mⅱmouse oocytes and second polar body formation (PBF) was examined. Suporose fraction RV2.10 prepared from sperm extract significantly increased PBF. Of Superdex fractions re-separated from Suporose fraction RV2.10, fraction RV2.12 also had the strongest PBF activity. By analyzing with micro-reverse phase column (URPC), the Superdex fraction RV2.12 appeared to be glutamic acid. In microinjection test, glutamic acid significantly increased PBF. This study suggests that glutamic acid should be a type of sperm factor for second polar body formation related to oocyte activation.
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KEYWORD
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Oocyte activation, Second polar body, Sperm factor, Glutamic acid
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